LOCUS       40924_45903        6679 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVC7N DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Hashiro S, Yasueda H.
  TITLE     High copy number mutants derived from Corynebacterium glutamicum 
            cryptic plasmid pAM330 and copy number control
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Hashiro S, Yasueda H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2018) Contact:Shuhei Hashiro AJINOMOTO CO., INC., 
            Frontier Research Labs. Institute For Innovation; 1-1 Suzuki-Cho, 
            Kawasaki-Ku, Kawasaki, Kanagawa 210-8681, Japan URL 
            :http://www.ajinomoto.com/
REFERENCE   3  (bases 1 to 6679)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6679)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-SEP-2018) Contact:Shuhei Hashiro AJINOMOTO CO., INC., Frontier 
            Research Labs. Institute For Innovation; 1-1 Suzuki-Cho, 
            Kawasaki-Ku, Kawasaki, Kanagawa 210-8681, Japan URL 
            :http://www.ajinomoto.com/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..4451
                     /label=pAM330N region
                     /note="pAM330N region"
     CDS             1215..2600
                     /codon_start=1
                     /gene="repA"
                     /label=repA
                     /protein_id="BBG56731.1"
                     /translation="MTTIALAKTTGTTPAQTRTAQPRTTSGLGNTEIEVNTSKEPQVNE
                     GSKVTRARAWRRQNVMYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRW
                     GSPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSS
                     MVKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSM
                     FSRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASK
                     GSYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDA
                     DVRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKD
                     VAAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTIL
                     RTH"
     gene            1215..2600
                     /gene="repA"
                     /label=repA
     CDS             4545..5201
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     protein_bind    5413..5434
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5449..5479
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5487..5503
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5511..5527
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(5540..5596)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5597..5613)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(5939..6527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcttgtcta cgtctgatgc tttgaatcgg acggacttgc cgatcttgta tgcggtgatt
       61 tttccctcgt ttgcccactt tttaatggtg gccggggtga gagctacgcg ggcggcgacc
      121 tgctgcgctg tgatccaata ttcggggtcg ttcactggtt cccctttctg atttctggca
      181 tagaagaacc cccgtgaact gtgtggttcc gggggttgct gatttttgcg agacttctcg
      241 cgcaattccc tagcttaggt gaaaacacca tgaaacacta gggaaacacc catgaaacac
      301 ccattagggc agtagggcgg cttcttcgtc tagggcttgc atttgggcgg tgatctggtc
      361 tttagcgtgt gaaagtgtgt cgtaggtggc gtgctcaatg cactcgaacg tcacgtcatt
      421 taccgggtca cggtgggcaa agagaactag tgggttagac attgttttcc tcgttgtcgg
      481 tggtggtgag cttttctagc cgctcggtaa acgcggcgat catgaactct tggaggtttt
      541 caccgttctg catgcctgcg cgcttcatgt cctcacgtag tgccaaagga acgcgtgcgg
      601 tgaccacgac gggcttagcc tttgcctgcg cttctagtgc ttcgatggtg gcttgtgcct
      661 gcgcttgctg cgcctgtagt gcctgttgag cttcttgtag ttgctgttct agctgtgcct
      721 tggttgccat gctttaagac tctagtagct ttcctgcgat atgtcatgcg catgcgtagc
      781 aaacattgtc ctgcaactca ttcattatgt gcagtgctcc tgttactagt cgtacatact
      841 catatttacc tagtctgcat gcagtgcatg cacatgcagt catgtcgtgc taatgtgtaa
      901 aacatgtaca tgcagattgc tgggggtgca gggggcggag ccaccctgtc catgcggggt
      961 gtggggcttg ccccgccggt acagacagtg agcaccgggg cacctagtcg cggatacccc
     1021 ccctaggtat cggacacgta accctcccat gtcgatgcaa atctttaaca ttgagtacgg
     1081 gtaagctggc acgcatagcc aagctaggcg gccaccaaac accactaaaa attaatagtt
     1141 cctagacaag acaaaccccc gtgcgagcta ccaactcata tgcacggggg ccacataacc
     1201 cgaaggggtt tcaattgaca accatagcac tagctaagac aacgggcaca acacccgcac
     1261 aaactcgcac tgcgcaaccc cgcacaacat cgggtctagg taacactgaa atagaagtga
     1321 acacctctaa ggaaccgcag gtcaatgagg gttctaaggt cactcgcgct agggcgtggc
     1381 gtaggcaaaa cgtcatgtac aagatcacca atagtaaggc tctggcgggg tgccataggt
     1441 ggcgcaggga cgaagctgtt gcggtgtcct ggtcgtctaa cggtgcttcg cagtttgagg
     1501 gtctgcaaaa ctctcactct cgctgggggt cacctctggc tgaattggaa gtcatgggcg
     1561 aacgccgcat tgagctggct attgctacta agaatcactt ggcggcgggt ggcgcgctca
     1621 tgatgtttgt gggcactgtt cgacacaacc gctcacagtc atttgcgcag gttgaagcgg
     1681 gtattaagac tgcgtactct tcgatggtga aaacatctca gtggaagaaa gaacgtgcac
     1741 ggtacggggt ggagcacacc tatagtgact atgaggtcac agactcttgg gcgaacggtt
     1801 ggcacttgca ccgcaacatg ctgttgttct tggatcgtcc actgtctgac gatgaactca
     1861 aggcgtttga ggattccatg ttttcccgct ggtctgctgg tgtggttaag gccggtatgg
     1921 acgcgccact gcgtgagcac ggggtcaaac ttgatcaggt gtctacctgg ggtggagacg
     1981 ctgcgaaaat ggcaacctac ctcgctaagg gcatgtctca ggaactgact ggctccgcta
     2041 ctaaaaccgc gtctaagggg tcgtacacgc cgtttcagat gttggatatg ttggccgatc
     2101 aaagcgacgc cggcgaggat atggacgctg ttttggtggc tcggtggcgt gagtatgagg
     2161 ttggttctaa aaacctgcgt tcgtcctggt cacgtggggc taagcgtgct ttgggcattg
     2221 attacataga cgctgatgta cgtcgtgaaa tggaagaaga actgtacaag ctcgccggtc
     2281 tggaagcacc ggaacgggtc gaatcaaccc gcgttgctgt tgctttggtg aagcccgatg
     2341 attggaaact gattcagtct gatttcgcgg ttaggcagta cgttctagat tgcgtggata
     2401 aggctaagga cgtggccgct gcgcaacgtg tcgctaatga ggtgctggca agtctgggtg
     2461 tggattccac cccgtgcatg atcgttatgg atgatgtgga cttggacgcg gttctgccta
     2521 ctcatgggga cgctactaag cgtgatctga atgcggcggt gttcgcgggt aatgagcaga
     2581 ctattcttcg cacccactaa aagcggcata aaccccgttc gatattttgt gcgatgaatt
     2641 tatggtcaat gtcgcggggg caaactatga tgggtcttgt tgttgacaat ggctgatttc
     2701 atcaggaatg gaactgtcat gctgttatgt gcctggctcc taatcaaagc tggggacaat
     2761 gggttgcccc gttgatctga tctagttcgg attggcgggg cttcactgta tctgggggtg
     2821 gcatcgtgaa tagattgcac accgtagtgg gcagtgtgca caccatagtg ggcatgagta
     2881 atacctacgc gcgcgtgggc tagggcttaa cgcgcgtttt gccgtgctgc ggggcatacg
     2941 ttagcgcata cgcttttttc tgtgaaacct ttttgtgttg ttgtttcgtg ttggtttcct
     3001 ttctgttggc ggggcaactt aacgcctgcg ggggtggttg ttgacgttaa cgggggtagt
     3061 ttttattccc ctagtggttt ttcagtacga caatcgagaa agacctgttt cagccagttc
     3121 gggtcatgtt cgtcggtatg gccacgtgca tagcgaccag ttttcgagtt cactgggatt
     3181 tttggtgcat cgaacaagat gtaggacaat gcggtttcta ggtctacttt ttgctttatg
     3241 ccgtacaagc cccgtgggta ttcagcgatt gattccaagg cggcttccca gtcctgtttt
     3301 gtgaaggact ggcttagttc taggtctgtg tctgggtagt actgcttgtt tgtgtaagcg
     3361 ccgttggtgc tcattgatga ttcctttgaa gtgtttggag ttcggctagt agtgcggcgt
     3421 atggtgctgc tttttgctcg tgatagctcg ccttggctat gaggtcggct aggtaggttt
     3481 ccggggtgcc taggttgcgt aggtctagca aatcccggta tgtggcctgt gcgctgcgct
     3541 ggtggtgcat acagtcgtta agctgggctt ttacgtctgc gatgcggtgg cggttaggca
     3601 tgttggtgtg cttcttccaa gtactcacgg gcgggttttg tgtatgcctg gcgtgatgct
     3661 tctttgagct gttggagttc cgcttggagt gcgggtagtt cgtccgcgaa ctgcttgtgg
     3721 tactcgtatt tctcttgttc ctgggcgata gcatttgcgt tgaattgcag ggcggtgagt
     3781 tcgtccacgc gtcgttttgc tgcgttggtc atggtggcgt gccatttgcg gttgtggacg
     3841 cggggttcaa ggttgcgcac ggctgcttcg gctaggttgg tggctgcttt tttcagtgct
     3901 cgggcttccc gttcctcgtc caacgagagc acctttggtt tgttggcttc ggctagtttt
     3961 tgcttctccg ctttgatgag ttggtcaact tcgtgttggg agaggtcgtt tttcacgatg
     4021 cgtcgaatgt ggtcgttgtg ggtgctgagt tggtgtgaga ggtagtgggg ttctgggatt
     4081 tcggcgagtt ggtcgaggtt ggtgtagtgc gggttgcggc ctggttggtt gggttcgctg
     4141 gggaggtcga tgtatccggt tgagtctccg gcgtggttga agtgaattag gcgttggtag
     4201 ccgtattcct ggttggggag gtacgacaga atgaggaagt ttggtgcttc tcctgcaatg
     4261 agtcgtgcgt gttcgtagtt cggtactggg tcgtgctcgg ggagaatgtt cttttgggtc
     4321 atggcttctc tttctgttgc tctgtaagtc cgtatgtggg catgggaaag ccccggcaac
     4381 cctttgggtc aaccggggct agatagtcgc ttagaatggc ttctaggctg cgtctcgggg
     4441 tgtggcaagc tgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     4501 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     4561 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     4621 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     4681 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     4741 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     4801 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     4861 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     4921 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     4981 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     5041 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     5101 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     5161 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
     5221 attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg
     5281 gcagaaattc agcttggccc agtgccaagc tccaatacgc aaaccgcctc tccccgcgcg
     5341 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     5401 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     5461 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5521 ctatgaccat gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg
     5581 taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     5641 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     5701 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgagcttctt
     5761 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5821 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5881 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6061 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6121 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6181 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6241 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6301 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6361 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6421 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6481 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6541 tcttttctac ggggtctgac gctcagtgga actccgtcga acggaagatc acttcgcaga
     6601 ataaataaat cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa
     6661 atgagacgtt gatcggcac
//
